Nutritional Neuroscience, Volume (23), No (5), Year (2020-5) , Pages (343-352)

Title : ( Vitamin K2 protects PC12 cells against Aβ (1-42) and H2O2-induced apoptosis via p38 MAP kinase pathway )

Authors: Elham Hadipour , Z. Tayarani-Najaran , Masoud Fereidoni ,

Citation: BibTeX | EndNote

Abstract

Alzheimer’s disease is an age-related disease with a hallmark of progressive loss of memory formation followed by a damage in the brain function due to the neural degeneration and extracellular beta-amyloid (Aβ) plaques accumulation. This study examines the protective effects of vitamin K2 on toxicity induced by (Aβ) (1-42) and H2O2 in PC12 cells as an appropriate model of Alzheimer’s cell damage. PC12 cells pretreated with vitamin K2 (5-200 μM) for 4, 24 and 48 h, and exposed to either Aβ (25 µM) for 48 h or H2O2 (150 µM) for 24 h. Then the protective, antioxidant and anti-apoptotic effects of vitamin K2 in PC12 cells were investigated. Vitamin K2 pretreatment (5-200 μM) significantly decreased the Aβ (1-42) and H2O2 cytotoxicity. In addition, vitamin K2 could attenuate reactive oxygen species (ROS) level after exposure of cells to H2O2 for 24 h and Aβ (1-42) for 48 h. Cell apoptosis significantly increased following application of Aβ (1-42) (25 μM) and H2O2 (150 µM) compared to control. However, flow cytometry histograms of PI-stained cells after pretreatment with vitamin K2 (20 and 50 μM) showed significantly reduced apoptosis. Vitamin K2 increased the amount of glutathione after exposure of cells to H2O2 for 24 h and Aβ (1-42) for 48 h. Western blot analysis of PC12 cells showed that 25 μM Aβ (1-42) and 150 µM H2O2 treatment could increase Bax, PARP cleavage, Phospho-p38 MAPK. Moreover, the activated form of caspase 3 proteins led to the reduction in the Bcl-2. Real-time PCR of PC12 cells showed that 150 µM H2O2 treatment increased the ratio of Bax/Bcl-2 while vitamin K2 (20 and 50 μM) reduced the rate. According to these findings, it seems that vitamin K2 possess anti-apoptotic and antioxidant effects and suggests that vitamin K2 may be a valuable protective candidate against the progression of Alzheimer’s disease via inactivating p38 MAP kinase pathway.

Keywords

, Alzheimer’s disease; Antioxidant; Beta, amyloid; Vitamin K2
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@article{paperid:1069671,
author = {Hadipour, Elham and Z. Tayarani-Najaran and Fereidoni, Masoud},
title = {Vitamin K2 protects PC12 cells against Aβ (1-42) and H2O2-induced apoptosis via p38 MAP kinase pathway},
journal = {Nutritional Neuroscience},
year = {2020},
volume = {23},
number = {5},
month = {May},
issn = {1028-415X},
pages = {343--352},
numpages = {9},
keywords = {Alzheimer’s disease; Antioxidant; Beta-amyloid; Vitamin K2},
}

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%0 Journal Article
%T Vitamin K2 protects PC12 cells against Aβ (1-42) and H2O2-induced apoptosis via p38 MAP kinase pathway
%A Hadipour, Elham
%A Z. Tayarani-Najaran
%A Fereidoni, Masoud
%J Nutritional Neuroscience
%@ 1028-415X
%D 2020

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