Background and Aim:Nowadays bacterial cellulose (BC) because of its outstanding properties and increased applications has become more attractive than plant cellulose. However one of the major issues in BC production is the expenses of substrates (carbon and nitrogen sources). Biowastes such as plant wastes are incredible resources for many industrial products. Therefore in this research beet molasses were used for BC production. Methods:Acetobacter xylinum BPR2001 was used for BC production as main bacterial strain. Different ratios of beet molasses and CSL were designed in 27 experiments. Experiments were included with the 50 ml/L of diluted beet molasses in water (1:4 ratio) in combination with 10 ml/L CSL (without diluting, diluted in the ratios of 1:1 and 2:1) in 3 levels (non-pretreatment, heat- pretreatment, H2SO4 and heat pretreatment). Parameters such as pH 6, ethanol 2% and bacterial inoculation quantity 10% were the same for all experiments. All experiments were conducted in triplicate at 28oC for 7 days in static cultures. Results:Outcomes revealed that the highest amount of dry BC (5.1 g/L) can be produced from the media of heat-pretreatment molasses and non-pretreated undiluted CSL. Also the amount of 4.63 g/L dry BC was obtained from the heat-pretreatment molasses with H2SO4 and heat pretreated CSL (2:1) media. Finally, in Hestrin-Shramm standard medium with the same conditions the amount of 4 g/L dry BC was produced. Conclusion:Pretreatment of molasses and CSL with heat and H2SO4 could improve the bacterial cellulose production as a beneficial and efficient method.

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