Many works of the past and the history of different tribes can be found in the monographs and the manuscript. For this reason, this kind of works are of great value. The microorganisms on these kind of written sources, not only cause complications such as allergies and skin and respiratory diseases which have a harmful effect on human health, biodeteriorate them. Astane Qudse Razavi library and document center; was known as one of the richest centers and repositories of written works in Iran. The aim of this study was to investigate the initial microbial pathology of some manuscripts in this center and to detect the microbial contaminant in the air of its reservoir. For this reason, sampling of written works was carried out using sterile nitrocellulose paper and swab.Then, the air of different areas of the reservoir was sampled via sedimentation method and cultured onto the some culture media. Based on macroscopic and microscopic characteristics, and some biochemical tests; fungi and bacteria were partially identified. Investigations showed that the isolated fungi in terms of their frequency, were Penicillium.sp, Cladosporium.sp, Alternaria.sp, Aspergillus.sp, Rhizopus.sp, Cephalosporium.sp and yeasts. Gram-positive basilli,gram-positive cocci, gram-negative bacilli and actinomycetes based on their frequency were isolated, respectively from the written works and air. The role of these microorganisms on the deteriorated paper and leather should be investigated. Furthermore;detection of airborne contaminating microorganisms in the air can improve the ventilation protocols of these reservoirs. Identification of these microbial agents will help conservator and restorators to select its efficient method.

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