10th International Symposium on the Nutrition of Herbivores , 2018-09-02

Title : ( Effect of using RumenBuff plus® on in vitro duodenal utilizable crude protein of two glucogenic diets )

Authors: BEHNAZ EYNI , Mohsen Danesh Mesgaran ,

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Abstract

Proceedings of the 10th International Symposium on the Nutrition of Herbivores 2018 Herbivore nutrition supporting sustainable intensification and agro‐ecological approaches Advances in Animal Biosciences This book is part of a series, which is a companion to the journal ANIMAL 2 to 6 September 2018, Clermont‐Ferrand, France 387 Session 2: Microbial ecosystem and herbivore nutrition Effect of using RumenBuff plus® on in vitro duodenal utilizable crude protein of two glucogenic diets Behnaz Eyni, Mohsen Danesh mesgaran, Foad Garajian Ferdowsi university, Mashhad, Iran E-mail: behnaz_eyni@yahoo.com Take home message Dietary buffers have been added to dairy cattle diets in an attempt to improve ruminal fermentation. Introduction When animal production increases, additional protein must be supplied from dietary protein that passes the rumen undegraded to meet the animal’s protein requirement. Therefore, it is important to maximize amino acid reach in the small intestine. Administration of a dietary buffers improve protein digestibility wich could increase milk yield, milk fat percentage, and optimize DMI in the lactating cow (Iwaniuk and Erdman, 2015). There are little data available about the effect of buffers on nitrogen flow to the small intestine. We hypothesized that exogenous dietary buffers, such as RumenBuff plus®, can be added to high grain diets and improve rumen fermentation and prevent acidosis. Therefore, the objective of our study was to measure the effects of exogenous dietary buffers in glucogenic diets on nitrogen flow to the small intestine of dairy cows. Materials & methods Two glucogenic diets based on barley grain (treatment 1 without additive) and (treatment 2 glucogenic diet based on barley grain + RumenBuff plus®) were analyzed for uCP using in vitro gas production technique. The procedure of in vitro CP utilization in duodenum was carried out using the gas production system (Edmunds et al., 2012) in four replicates and three runs. The utilization of CP in duodenum was calculated as the difference between the N concentrations in the sample plus the ammonia concentration in the blank minus the ammonia-N concentration produced from the sample divided by the sample weight, using the following calculation: uCP (g/kg DM) = (NH3Nblank+ Nsample – NH3 Nsample)/(weight (mg DM)) ×6.25×1000, where the Nsample is N added to the bottles. Results & discussion In 8 and 24 hours after incubation, uCP tended to be higher in treatment containing RumenBuff plus®. Each factor affecting the quantity of the microbial protein leaving the rumen has the ability to influence uCP (Bach et al., 2000). Probably, the positive effect of RumenBuff plus® on rumen fermentation is the result of increasing microbial nitrogen flow to the small intestine. Utilizable crude protein after 48 h of incubation was not significantly affected by treatments. Table 1: Effect of treatments on utilizable crude protein (uCP) after 8, 24 and 48 h of incubation. SEM treatment* Time (h) 1 2 8 151.5b 219.4a 20.9 uCP(g/kg DM) 24 122.2b 131.5a 16.6 48 75.7 75.8 2.4 * treatment 1: glucogenic diet without addition, treatment 2: glucogenic diet with Rumen Buff plus® Conclusion Results indicate that Rumen Buff plus ® could be used in dairy cow’s ration to improve fermentation and increase nitrogen flow to the small intestine. References Bach A, Huntington GB, Calsamiglia S and Stern MD 2000. Journal of Dairy Science 83, 2585–2595. Edmunds B, Sudeku KH, Spiekers H, Schusterc M and Schwarzd FJ 2012. Animal Feed Science and Technology 175, 106– 113. Iwaniuk ME and Erdman RA 2015. Journal of Dairy Science 98, 1–13.

Keywords

glucogenic diets
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@inproceedings{paperid:1071765,
author = {EYNI, BEHNAZ and Danesh Mesgaran, Mohsen},
title = {Effect of using RumenBuff plus® on in vitro duodenal utilizable crude protein of two glucogenic diets},
booktitle = {10th International Symposium on the Nutrition of Herbivores},
year = {2018},
location = {IRAN},
keywords = {glucogenic diets},
}

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%0 Conference Proceedings
%T Effect of using RumenBuff plus® on in vitro duodenal utilizable crude protein of two glucogenic diets
%A EYNI, BEHNAZ
%A Danesh Mesgaran, Mohsen
%J 10th International Symposium on the Nutrition of Herbivores
%D 2018

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