Acetaminophen (APAP) toxicity may be the consequence of either an acute overdose [1]. Therapeutic levels are 5-20 μg/mL (33-132 μmol/L) [2]. various chromatographic techniques, have proved to be reliable and accurate methods for quantifying APAP concentrations in biological samples [3]. However, these approaches require complex sample preparation procedures, expensive and bulky instruments, and professionally trained personnel running the tests. Therefore, they are not well suited for rapid on-site detection of APAP and may not even be available for use in developing countries. Resently, gold nanoparticle (Au NP)-based colorimetric assays are emerging as alternative approaches for detection, providing high sensitivity, specificity, and ease of signal read-out. So, in this work we used POM-(polyoxometalate) modified gold nanoparticles that synthesized by Sonochemistry method in different temperature, potential and the proportions chloroauric acid and POM. Colorimetry was performed by a digital camera and the data was obtained by the Photoshop CC 2017 software. The method was optimized for effect of pH, reaction time, volume ratio of POM-Au NP, ambient light condition and light intensity. Under optimized condition linear range and limit of detection are 5-40 mg/L, 0.5 mg/l respectively. It is important to develop a quick, simple, reliable and sensitive method, which can detect APAP. We have effectively established a novel nanosensor with high sensitivity for the visual and quantitative detection of APAP in serum from animal samples.

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