In the present study, multistep formation mechanism and synthesis reaction pathway of therapeutic magnetite nanoplatforms during a modified chemical coprecipitation method were identified. Critical reaction steps were considered through the study of pH variation trend as a function of structural, microstructural, and magnetic properties. Structural, microstructural, and magnetic characteristics were investigated using XRD, FT-IR, DLS, TEM, and VSM techniques. Results demonstrate the synthesis pathway of magnetite nanoparticles significantly depends on reaction pH and could be deduced in four successive transformation steps as follows: -I- initial materials to ferrous hydroxide, -II- ferrous hydroxide to lepidocrocite phase, -III- lepidocrocite to goethite phase, and -IV- goethite to magnetite -or/and maghemite- phase. Moreover, aqueous ferrofluids of synthesized Fe3O4 nanoparticles demonstrate significant heat-generation ability and SAR values upon applying AC magnetic field which confirm their appropriate efficiency to utilize for therapeutic hyperthermia applications.

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