Biochimie, Volume (163), No (1), Year (2019-8) , Pages (84-93)

Title : ( Linkage between EMT and stemness state through molecular association between TWIST1 and NY-ESO1 in esophageal squamous cell carcinoma )

Authors: Sima Ardalan Khales , Mohammad Reza Abbaszadegan , Ahmad Majd , Mohammad Mahdi Forghanifard ,

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Abstract

Aberrant expression of cancer testis antigens (CTAs) is reported in tumors, especially those with stemness properties. A number of CTAs can induce epithelial mesenchymal transition (EMT) process and promote cancer stem cells (CSCs) characteristics. We aimed in this study to analyze the correlation between NY-ESO1 and TWIST1 in esophageal squamous cell carcinoma (ESCC), as well as their impact on EMT process. Gene expression profiling of NY-ESO1 and TWIST1 was performed in 43 esophageal tumors compared to their margin normal tissues of using qRT-PCR, and their correlation with clinicopathological variables of the patients was evaluated. In silico analysis of the NY-ESO1, epithelial and mesenchymal cell markers and also their promoter sequences was executed. ESCC cell lines KYSE-30 and YM-1 were transduced to ectopically express TWIST1 using a retroviral system, followed by qRT-PCR mRNA expression analysis to reveal the probable correlation among TWIST1, NY-ESO1 and EMT markers gene expression. Scratch assay was performed to estimate migration of TWIST1-induced cells. Overexpression of TWIST1 and NY-ESO1 mRNA was observed in 42% and 39.5% (P ˂ 0.05) of tumors, respectively. Expression of the genes was significantly correlated with each other (p = 0.005). TWIST1 and NY-ESO1 overexpression was significantly associated with stage of progression and size of tumors, respectively. A direct association between TWIST1 and NY-ESO1 mRNA expression was confirmed by induced ectopic expression of TWIST1 in ESCC cell lines KYSE-30 and YM-1. TWIST1-induced cells led to increase migration in ESCC cell line. Furthermore, significant up-regulation of EMT markers was observed following ectopic expression of TWIST1 in these cells. Based on our findings, it may be proposed that a vital association is exist between the EMT and the acquisition of cancer stemness state in tumor cells through the TWIST1/NY-ESO1 axis and it can be a critical hallmark in ESCC tumorigenesis.

Keywords

, NY, ESO1 TWIST1 Epithelial mesenchymal transition Mesenchymal stem cell Esophageal squamous cell carcinoma
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@article{paperid:1074799,
author = {Sima Ardalan Khales and Mohammad Reza Abbaszadegan and Ahmad Majd and Mohammad Mahdi Forghanifard},
title = {Linkage between EMT and stemness state through molecular association between TWIST1 and NY-ESO1 in esophageal squamous cell carcinoma},
journal = {Biochimie},
year = {2019},
volume = {163},
number = {1},
month = {August},
issn = {0300-9084},
pages = {84--93},
numpages = {9},
keywords = {NY-ESO1 TWIST1 Epithelial mesenchymal transition Mesenchymal stem cell Esophageal squamous cell carcinoma},
}

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%0 Journal Article
%T Linkage between EMT and stemness state through molecular association between TWIST1 and NY-ESO1 in esophageal squamous cell carcinoma
%A Sima Ardalan Khales
%A Mohammad Reza Abbaszadegan
%A Ahmad Majd
%A Mohammad Mahdi Forghanifard
%J Biochimie
%@ 0300-9084
%D 2019

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