Life Sciences, ( ISI ), Volume (232), No (232), Year (2019-9) , Pages (116661-116661)

Title : ( Polyethylenimine-associated cerium oxide nanoparticles: A novel promising gene delivery vector )

Authors: Leila Hasanzadeh , Majid Darroudi , Navid Ramezanian , Parvin Zamani , Seyed Hamid Aghaee-Bakhtiari , Esmail Nourmohammadi , Reza Kazemi Oskuee ,

Citation: BibTeX | EndNote

Abstract

Aims: The development of highly efficient and low toxic non-viral gene delivery vectors is the most challenging issues for successful application of gene therapy. A particular focus has been on understanding structure-activity relationships for transfection activity and toxicity of polyethylenimine (PEI). During the last decade, the use of cerium oxide nanoparticles (CeO2-NPs) in biomedicine has attracted much attention due to their pH-dependent antioxidant activity. CeO2-NPs provide protection normal cells from various forms of reactive oxygen species, but possess innate cytotoxicity and apoptosis to cancer cells. The purpose of this study was to design a new class of gene carriers by low molecular weight PEI (B-PEI 10kDa) coordination onto CeO2-NPs. Main methods: B-PEI 10kDa was conjugated to CeO2-NPs by Epichlorohydrin linker. Transfection efficiency, cytotoxic and apoptotic effects of pDNA-PEI-CeO2 NPs were evaluated on WEHI 164 cancer cells and normal L929 cells lines. Key findings: PEI-CeO2 NPs was able to condense the pDNA at carrier/plasmid (C/P) weight ratios of 0.5. The size and zeta potential of pDNA-PEI-CeO2 NPs were 124 ± 7nm and 22 ± 2mV, respectively. The transfection efficacy of synthesized pDNA-PEI-CeO2 NPs improved and the cytotoxicity was decreased compared to pDNAPEI. Moreover, pDNA-PEI-CeO2 NPs induced more apoptosis than unmodified PEI and CeO2-NPs control groups. pDNA-PEI-CeO2 NPs displayed more transfection, cytotoxicity, and apoptosis in WEHI 164 cancer cells than normal L929 cells. Significance: In conclusion, PEI-CeO2 nanocarriers could act as a potential candidate for gene and drug delivery to cancerous and tumor cells.

Keywords

Cerium oxide nanoparticles Polyethylenimine Gene delivery Transfection Cytotoxicity
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@article{paperid:1075120,
author = {Leila Hasanzadeh and Majid Darroudi and Ramezanian, Navid and Parvin Zamani and Seyed Hamid Aghaee-Bakhtiari and Esmail Nourmohammadi and Reza Kazemi Oskuee},
title = {Polyethylenimine-associated cerium oxide nanoparticles: A novel promising gene delivery vector},
journal = {Life Sciences},
year = {2019},
volume = {232},
number = {232},
month = {September},
issn = {0024-3205},
pages = {116661--116661},
numpages = {0},
keywords = {Cerium oxide nanoparticles Polyethylenimine Gene delivery Transfection Cytotoxicity},
}

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%0 Journal Article
%T Polyethylenimine-associated cerium oxide nanoparticles: A novel promising gene delivery vector
%A Leila Hasanzadeh
%A Majid Darroudi
%A Ramezanian, Navid
%A Parvin Zamani
%A Seyed Hamid Aghaee-Bakhtiari
%A Esmail Nourmohammadi
%A Reza Kazemi Oskuee
%J Life Sciences
%@ 0024-3205
%D 2019

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