Iranian Journal of Basic Medical Sciences, ( ISI ), Volume (22), No (9), Year (2019-6) , Pages (1026-1035)

Title : ( Brevinin-2R-linked polyethylenimine as a promising hybrid nanogene- delivery vector )

Authors: Fatemeh Zohrab , Ahmad Asoodeh , Amin Jalili , Majid Darroudi , Reza Kazemi Oskuee ,

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Abstract

Objective(s): Polyethylenimine (PEI) is one of the most widely used polymers in gene delivery. The aim of this study was to modify PEI by replacing some of its primary amines with Brevinin 2R (BR-2R) peptide in order to increase the efficiency of gene delivery. Materials and Methods: Polyethylenimine was modified by BR-2R peptide by two different approaches; A) conjugation methods including (І) using succinimidyl 3-(2-pyridyldithio) propionate (SPDP), (П) EDC/ NHS protocol and (ПІ) EDC/NHS+6-bromohexanoic acid protocol, and B) physical interaction method. The modified polymers were characterized for their ability of plasmid condensation, number of primary amines, size and zeta potential. The transfection efficiency and cytotoxicity were evaluated on HEK293, L929, WEHI164 and Neuro2A cell lines by green fluorescent protein (GFP)-based plasmid (pGFP) reporter gene and viability assays, respectively. Apoptosis induction ability was also evaluated via PI/Annexin V assay. Results: Polyplex had size and zeta potential between 200-270 nm and +21.5- +28.4 mV, respectively. All vectors were able to condense plasmid DNA in C/P=4 (carrier-plasmid ratio). Transfection results on the Neuro2A cell line showed that the vector containing the BR-2R peptide, which was synthesized using EDC-NHS protocol had the best transfection efficiency. Conclusion: Our results showed that conjugation of Brevinin 2R as cell penetrating peptide to polyethyleneimine could enhance the transfection ability of the polymer.

Keywords

Brevinin 2R Cell penetrating peptide Gene delivery Polyethyleneimine Vector
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@article{paperid:1075269,
author = {Fatemeh Zohrab and Asoodeh, Ahmad and امین جلیلی and مجید درودی and رضا کاظمی اسکویی},
title = {Brevinin-2R-linked polyethylenimine as a promising hybrid nanogene- delivery vector},
journal = {Iranian Journal of Basic Medical Sciences},
year = {2019},
volume = {22},
number = {9},
month = {June},
issn = {2008-3866},
pages = {1026--1035},
numpages = {9},
keywords = {Brevinin 2R Cell penetrating peptide Gene delivery Polyethyleneimine Vector},
}

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%0 Journal Article
%T Brevinin-2R-linked polyethylenimine as a promising hybrid nanogene- delivery vector
%A Fatemeh Zohrab
%A Asoodeh, Ahmad
%A امین جلیلی
%A مجید درودی
%A رضا کاظمی اسکویی
%J Iranian Journal of Basic Medical Sciences
%@ 2008-3866
%D 2019

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