Iranian Journal of Biotechnology, ( ISI ), Volume (17), No (2), Year (2019-6) , Pages (55-60)

Title : ( Cultivation Effect of Chitinase-Transgenic Cotton on Functional Bacteria and Fungi in Rhizosphere and Bulk Soil )

Authors: zahra sadat shahmoradi , Masoud Tohidfar , Hassan Marashi , Saeid Malekzadeh Shafaroudi , Ebrahim Karimi ,

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Abstract

Background: In consideration for the increasing widespread use of genetically modified (GM) crops, one of the important issues for assessment is the effect of GM crops on soil microbial communities Objectives: In this study, T2 chitinase-transgenic cotton (line #57) and its non-transgenic line were investigated for bacterial and fungal dynamics during its development stages. Material and Methods: The assessments were performed by viable plate count and polymerase chain reactiondenaturing gradient gel electrophoresis (PCR-DGGE) assays. Results: Viable plate count analysis showed an increase in community structures and the number of culturable bacteria in rhizosphere of both transgenic and non-transgenic cultivars as compared to bulk soil. PCR-DGGE confirmed results of viable plate count assays of the changes in bacterial and fungal communities for all cotton development stages in rhizosphere and bulk zones. No significant differences in number of functional bacteria were observed between rhizosphere soil of chitinase transgenic and non-chitinase transgenic cotton at one particular stage. Conclusions: The results indicated that T2 chitinase-transgenic cotton (line #57) might have no adverse effects on community structures and total number of culturable bacteria and fungi in the rhizosphere.

Keywords

, Microorganisms, Genetically-Modified; Microbiota; Rhizosphere
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@article{paperid:1075294,
author = {Shahmoradi, Zahra Sadat and مسعود توحیدفر and Marashi, Hassan and Malekzadeh Shafaroudi, Saeid and ابراهیم کریمی},
title = {Cultivation Effect of Chitinase-Transgenic Cotton on Functional Bacteria and Fungi in Rhizosphere and Bulk Soil},
journal = {Iranian Journal of Biotechnology},
year = {2019},
volume = {17},
number = {2},
month = {June},
issn = {1728-3043},
pages = {55--60},
numpages = {5},
keywords = {Microorganisms; Genetically-Modified; Microbiota; Rhizosphere},
}

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%0 Journal Article
%T Cultivation Effect of Chitinase-Transgenic Cotton on Functional Bacteria and Fungi in Rhizosphere and Bulk Soil
%A Shahmoradi, Zahra Sadat
%A مسعود توحیدفر
%A Marashi, Hassan
%A Malekzadeh Shafaroudi, Saeid
%A ابراهیم کریمی
%J Iranian Journal of Biotechnology
%@ 1728-3043
%D 2019

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