Herein, nucleophilic substitution reaction of pyrimidine derivatives on piperazine in ciprofloxacin resulted in the formation of new pyrimidyl N-ciprofloxacines. These compounds were prepared at room temperature, at conventional heating and under microwave irradiation conditions. Results indicated microwave condition as a green approach in comparison with room temperature and at conventional heating condition leads to higher yields and very short reaction times. 1H, 13C-NMR, FTIR, Mass spectroscopy and CHN analysis were used to characterize all the synthesized products. Meanwhile, the antibacterial activity of these new compounds against gram-positive and gram-negative bacteria was investigated and showed more activities for two new N-ciprofloxacin derivatives than ciprofloxacin.

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