Colorectal cancer as the second leading cause of cancer-related death with high prevalence have attracted scientists to unravel the secret of its underlying mechanisms. Although, novel therapeutics approaches like targeted therapy have shed the light on better responses, yet discovery of more effective biomarkers in order to early detection of new cases, introducing more effective therapeutic strategies and improving patient responses to therapy are required. Thus, targeting microRNAs that have pivotal roles in post-transcriptional regulation, especially miR-21 that is overexpressed in many cancers including colorectal cancer, can be a promising approach in cancer therapy. In the present study, the expression levels of miR-21 was evaluated in colon cancer cell line (RKO) and human embryonic kidney 293T cells following direct transfection of miRzip-anti-miR-21 via real-time PCR analysis. Results confirmed downregulation of miR-21 as a consequence of transfection. It could be concluded that HEK293T cells considering their proper features including low basal expression of miR-21, high transfection efficiency rate and favorable proliferation capacity are suitable means for transferring lots of intact anti-miR-21 to target cancerous cells with high expression level of miR-21.

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