Title : ( Isolation, purification and biochemical characterization alpha-amylase from microorganisms of gut extract of termite )
Authors: Samira Sargoli , Ahmad Asoodeh ,
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Abstract
Global enzyme market was attracted by α Amylases due to their vast applications in starch processing, detergent, alcohol, textile, food, paper, and pharmaceutical industries. Alpha-amylases can be obtained from various natural resources such as plants, animals, and microorganisms. The dominant source of amylase production are microorganisms due to optimum growth requirements, accessibility, efficient, eco-friendly, and cost-effective as compared to other resources such as animal and plant. This study reports the purification and characterization of a novel raw starch digesting α-amylase from a newly isolated Bacillus sp.CF96 of gastrointestinal tract termite. Maximum α-amylase activity was obtained at 37 ℃ after 36 h of incubation in incubator. We examined different nitrogen sources, temperature and pH to optimize produce more starch hydrolyzing α amylases. The amylase exhibited optimal activity and maximum growth rate in basal medium that contain peptone 1% as nitrogen source, pH 7 and 37 ℃.
Keywords
, α-Amylase, biochemical characterization, peptone, starch
@inproceedings{paperid:1075811,
author = {Sargoli, Samira and Asoodeh, Ahmad},
title = {Isolation, purification and biochemical characterization alpha-amylase from microorganisms of gut extract of termite},
booktitle = {چهارمین کنگره سالانه بین المللی توسعه کشاورزی،منابع طبیعی، محیط زیست و کشاورزی ایران},
year = {2019},
location = {تبریز, IRAN},
keywords = {α-Amylase; biochemical characterization; peptone; starch},
}
%0 Conference Proceedings
%T Isolation, purification and biochemical characterization alpha-amylase from microorganisms of gut extract of termite
%A Sargoli, Samira
%A Asoodeh, Ahmad
%J چهارمین کنگره سالانه بین المللی توسعه کشاورزی،منابع طبیعی، محیط زیست و کشاورزی ایران
%D 2019
