4th International Congress of Developing Agriculture, Natural Resources, Environment and Tourism of Iran , 2019-08-14

Title : ( Inhibitory effects of siRNA on Myostatin expression in C2C12 cells )

Authors: hengame payande ,

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Abstract

Myostatin is a growth-promoting factor that inhibits muscle growth during the myogenic process. When myostatin is active, cells quickly differentiate into muscle cells that prevent their growth. The inhibition of the expression of the gene expression of myostatin causes the growth of cells and prevents rapid differentiation. siRNA for silencing mouse myostatin gene was designed by E-RNAi software. This siRNA was designed to be able to inhibit the transcription of myostatin. A negative siRNA, which is not complement to any region of the mouse genome was used as control. SiRNA was transfected in C2C12 cells with Lipofectamine 3000. The myostatin gene expression was assessed in three periods of 24, 48 and 96 h after transfection. Three biological replicates were considered for each time. According to the results, siRNA had a significant effect on silencing myostatin gene by inhibiting transcription. Analysis of the expression data showed that RNAi was able to shut down the myostatin gene by more than 95% within 96 hours. The activity of the myostatin was estimated 10 and 15% at 48 and 24, respectively.

Keywords

, Myostatin, siRNA, Inhibition of gene expression, mouse myoblast