Geographical distribution of tick vectors of theilriosis and babesiosis in livestock in large Khorasan, Iran Gholamreza Razmi Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran, razmi@um.ac.ir Backgrounds: Babesiosis and theileriosis are important tick-borne diseases in Iran. Several molecular studies were carried out to identify tick vectors of Theilriosis and Babesiosis in cattle, sheep, goats and horses in different areas in large Khorasan from 2003- 2016. Methods: A total 3924 Ixodid ticks were collected from different areas of large Khorasan. The collected ticks were separated into tick pools, according to their species and sex. Then, their salivary glands and ovaries were dissected out in 0.85% saline under stereomicroscope and analyzed by semi-nested PCR. Results: Eleven Ixodid tick were identified as follow : Rhipicephalus turanicus 52.2 % , Hyalomma excavatum 19.9 %, Hyalomma anatolicum 12.52 %, Hyalomma marginatum 11.4% , Dermacentor marginatum 1.67%, , Rhipicephalus bursa 0.77, Hyalomma asiaticum 0.69, Dermacentor raskemensis 0.54, Dermacentor niveus 0.36%, Rhipicephalus sangieneus 0.12 and Haemaphysalis sulcata 0.05%. The genomic DNA of Theileria spp was detected in salivary glands of H.excavatum, H.anatolicum, H.marginatum, R. turanicus and R. bursa, while Babesia spp was detected in R.turanicus and H.marginatum Conclusion: The results suggest that H.excavatum could be as vector of T.annulata and T.equi, H.anatolicum as vector T.annulata and T.lestoquardi, H.marginatum and R. Turanicus as vector of T.ovis and B.ovis and R.bursa as vector of T.equi .

Keywords