Micobial Pathogenesis, ( ISI ), Volume (138), Year (2019-10) , Pages (103819-103826)

Title : ( Analysis of different signal peptides for the secretory production of Ama r 2 in gram-positive systems (Lactococcus lactis) )

Authors: Alireza Vasiee , Neda Norouzi , Farideh Tabatabaei yazdi , Sayed Ali Mortazavi , Mojtaba Sankian , Mahmoud Mahmoudi , Fakhri Shahidi ,

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Abstract

Prokaryotic systems have been considered the most affordable and simplest hosts which are being employed to express recombinant proteins such as allergens; nevertheless, without appropriate signal peptide (SP), these systems cannot be used for secretory proteins. Recently, a lot of effort has been put into assessing the potential of gram-positive strains such as lactic acid bacteria for new applications in the production of heterologous proteins. Ama r 2 is a respiratory allergen from Amaranthus retroflexus, whose recombinant production in the probiotic host could be introduced as a specific and effective way to rapid diagnosis and immunotherapy of this allergy. Consequently, the production of this recombinant protein using the prokaryotic system, requires a suitable SP to protect disulfide bonds and to prevent misfolding. This study was designed to predict the best SPs for the expression of Ama r 2 protein in Lactococcus lactis as the host. In this study, 42 signal sequences were selected from SP databases and the most important features of them were evaluated. First, n, h and c regions of the SPs and their probabilities were investigated by signalP software version 4.1. Then, their physicochemical properties were evaluated by Portparam and SOLpro. Moreover, the secretion sorting and sub-cellular localization sites were evaluated by PRED-TAT and ProtcompB software programs. The results revealed that yjgB, entC2 (Entrotoxine type C-2), ent B (Entrotoxine type), blaZ (Beta lactamase), dex (number 21), blm (Beta lactamase 2) dex (Dextranase; number 20) and number 26 were introduced theatrically as the best SPs to express Ama r 2 in Lactococcus lactis.

Keywords

Gram positive systemBioinformaticsAma r 2 pollenSignal peptide
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@article{paperid:1076508,
author = {Vasiee, Alireza and ندا نوروزی and Tabatabaei Yazdi, Farideh and Mortazavi, Sayed Ali and Mojtaba Sankian and محمود محمودی and Shahidi, Fakhri},
title = {Analysis of different signal peptides for the secretory production of Ama r 2 in gram-positive systems (Lactococcus lactis)},
journal = {Micobial Pathogenesis},
year = {2019},
volume = {138},
month = {October},
issn = {0882-4010},
pages = {103819--103826},
numpages = {7},
keywords = {Gram positive systemBioinformaticsAma r 2 pollenSignal peptide},
}

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%0 Journal Article
%T Analysis of different signal peptides for the secretory production of Ama r 2 in gram-positive systems (Lactococcus lactis)
%A Vasiee, Alireza
%A ندا نوروزی
%A Tabatabaei Yazdi, Farideh
%A Mortazavi, Sayed Ali
%A Mojtaba Sankian
%A محمود محمودی
%A Shahidi, Fakhri
%J Micobial Pathogenesis
%@ 0882-4010
%D 2019

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