Small mammals species are found based on the type of habitat and evolutionary past in particular areas. For this reason, specific field studies are required to understand the biodiversity of each region. The central Zagros area in Isfahan province due to its high habitat diversity is a good environment for studying small terrestrial mammals. In this study, two orders of rodentia and eulipotyphla have been studied. Sampling was done over a year using active and passive methods. A total of 108 samples were collected, of which 93 were rodents and 15 were insectivorous. Finally, based on morphological and cariological studies, 13 species of rodentia belong to 5 families of Muridae (Apodemus witherbyi, Mus musculus, Mus macedonicus, Meriones persicus), Gliridae (Dryomys nitedula), Cricetidae (Chionomys nivalis, Arvicola persicus, Ellobius lutescens, Microtus socialis, Cricetulus migratorius, Mesocricetus brandti), Dipodidae (Allactaga williamsi), Calomyscidae (Calomyscus), and two species of eulipotyphla belonging to two families of Soricidae (Crocidura suaveolens) and Erinacidae (Hemiechinus auritus). In this study, chromosomal characteristics of Chionomys nivalis and Crocidura suaveolens are reported for the first time in Iran. Also, the results of this study show the south distribution of Mus macedonicus to the central Zagros highlands in Iran. And also, the cariological and morphological studies of the Calomyscus population of the west of Isfahan province did not confirm the existence of the Calomyscus bailwardi in this area.

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