Journal of Physical Chemistry Letters, Volume (10), No (21), Year (2019-11) , Pages (6942-6947)

Title : ( Linear and Kinked Oligo(phenyleneethynylene)s as Ideal Molecular Calibrants for Förster Resonance Energy Transfer )

Authors: Martin F. Czar , Frauke D. Breitgoff , Dhananjaya Sahoo , Muhammad Sajid , Navid Ramezanian , Yevhen Polyhach , Gunnar Jeschke , Adelheid Godt , Renato Zenobi ,

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Abstract

We show that oligo(phenyleneethynylene)s (oligoPEs) are ideal spacers for calibrating dye pairs used for Förster resonance energy transfer (FRET). Ensemble FRET measurements on linear and kinked diads with such spacers show the expected distance- and orientationdependence of FRET. Measured FRET efficiencies match excellently with those predicted using a harmonic segmented chain model, which was validated by end-toend distance distributions obtained from pulsed electron paramagnetic resonance measurements on spin-labeled oligoPEs with comparable label distances.

Keywords

, Oligo(phenyleneethynylene)s , Förster Resonance Energy Transfer , end-toend distance distributions
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@article{paperid:1076704,
author = {Martin F. Czar and Frauke D. Breitgoff and Dhananjaya Sahoo and Muhammad Sajid and Ramezanian, Navid and Yevhen Polyhach and Gunnar Jeschke and Adelheid Godt and Renato Zenobi},
title = {Linear and Kinked Oligo(phenyleneethynylene)s as Ideal Molecular Calibrants for Förster Resonance Energy Transfer},
journal = {Journal of Physical Chemistry Letters},
year = {2019},
volume = {10},
number = {21},
month = {November},
issn = {1948-7185},
pages = {6942--6947},
numpages = {5},
keywords = {Oligo(phenyleneethynylene)s ; Förster Resonance Energy Transfer ; end-toend distance distributions},
}

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%0 Journal Article
%T Linear and Kinked Oligo(phenyleneethynylene)s as Ideal Molecular Calibrants for Förster Resonance Energy Transfer
%A Martin F. Czar
%A Frauke D. Breitgoff
%A Dhananjaya Sahoo
%A Muhammad Sajid
%A Ramezanian, Navid
%A Yevhen Polyhach
%A Gunnar Jeschke
%A Adelheid Godt
%A Renato Zenobi
%J Journal of Physical Chemistry Letters
%@ 1948-7185
%D 2019

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