The exercise-induced variations of some biochemical parameters including calcium (Ca), phosphorous (P), iron (Fe), copper (Cu), manganese (Mn), selenium (Se), zinc (Zn), sodium (Na), potassium (K), chlorine (Cl), glucose, urea, creatinine, lactate dehydrogenase (LDH), aspartate aminotransferase (AST), alanine aminotransferase (ALT), and creatine kinase (CK) were assessed in the serum of 25 Arabian horses. Blood samples were taken from the jugular vein 3 different times: before exercise, 5 h after exercise, and 18 h after exercise. Statistical analysis was performed using SAS (version 9.2; SAS Institute, Cary, NC, USA). Ca, Cl, glucose, and urea showed significant (P < 0.05) changes due to exercise. There was significant increase from before exercise to 5 h after exercise in urea concentrations while Ca and Cl concentrations significantly decreased. A significant ascending trend for Ca between 5 h and 18 h after exercise was also seen, while glucose and urea had a significantly descending trend. No significant time-based variations were observed for other variables. The results of the present study indicated that exercise could affect the value of some biochemical parameters, which could be used in future studies evaluating the health status of Arabian horses.

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