سومین همایش بین المللی و یازدهمین همایش ملی بیوتکنولوژی جمهوری اسلامی ایران , 2019-09-01

Title : ( Molecular identification and new host record for Cucumber mosaic virus infecting Ocimum basilicum in Iran )

Authors: HAYDER AL INIZI , Zohreh Moradi , Mohsen Mehrvar , Mohammad Zaki AGhl ,

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Abstract

Cucumber mosaic virus (CMV; genus Cucumovirus, family Bromoviridae) causes considerable losses in more than 1,200 plant species and is among the “top 10 plant viruses” due to heavy economic and yield loss. In June 2017, bush basil plants (Ocimum basilicum) showing virus-like symptoms, including mosaic and leaf distortion were observed in Sari county, Mazandaran province, in the north of Iran. Symptomatic leaf samples were collected, and sap was extracted from three infected leaf samples in 0.01 M potassium phosphate buffer, pH 7.2, and inoculated on to Ocimum basilicum. Plants were maintained in a glasshouse at 22 to 25°C with ∼70% relative humidity. After 15 days, the inoculated plants showed mosaic symptoms similar to those observed in the field. To diagnose virus infection in the collected samples, total RNA was extracted from both the original field samples and the symptomatic indicator plants using Qiagen RNeasy Plant Mini Kit. RT-PCR was performed using specific primer pairs CMV-CP-F (5′-ATGGACAAATCTGAATCAACC-3′) and CMV-CP-R (5′-TCAGACTGGGAGCACCCC-3′), which were designed to amplify a 657 bp fragment of the CMV coat protein gene. Amplified fragments of the expected size were purified, then cloned into pTG19-T vector and bi-directionally sequenced. A CMV isolate, IR1, was deposited in GenBank under accession number MK627936. A BLAST search in GenBank showed 97.33% nucleotide sequence identity with a Turkish CMV isolate (LC066503.1). To our knowledge, this is the first report of CMV naturally infection in bush basil in Iran.

Keywords

, CMV, bush basil, cloning, sequencing, Iran
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@inproceedings{paperid:1077629,
author = {AL INIZI, HAYDER and زهره مرادی and Mehrvar, Mohsen and Zaki AGhl, Mohammad},
title = {Molecular identification and new host record for Cucumber mosaic virus infecting Ocimum basilicum in Iran},
booktitle = {سومین همایش بین المللی و یازدهمین همایش ملی بیوتکنولوژی جمهوری اسلامی ایران},
year = {2019},
location = {تهران, IRAN},
keywords = {CMV; bush basil; cloning; sequencing; Iran},
}

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%0 Conference Proceedings
%T Molecular identification and new host record for Cucumber mosaic virus infecting Ocimum basilicum in Iran
%A AL INIZI, HAYDER
%A زهره مرادی
%A Mehrvar, Mohsen
%A Zaki AGhl, Mohammad
%J سومین همایش بین المللی و یازدهمین همایش ملی بیوتکنولوژی جمهوری اسلامی ایران
%D 2019

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