BMC Medical Genetics, ( ISI ), Volume (20), No (1), Year (2019-7)

Title : ( Overexpression of MicroRNA-148b-3p stimulates osteogenesis of human bone marrow-derived mesenchymal stem cells: the role of MicroRNA-148b-3p in osteogenesis )

Authors: Samaneh Mollazadeh , Bibi Sedigheh Fazly Bazzaz , Vajiheh Neshati , Antoine A. F. de Vries , Hojjat Naderi-Meshkin , Majid Mojarad , Mahdi Mirahmadi , Zeinab Neshati , Mohammad Amin Kerachian ,

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Abstract

Abstract BACKGROUND: Mesenchymal stem cells (MSCs) are attractive choices in regenerative medicine and can be genetically modified to obtain better results in therapeutics. Bone development and metabolism are controlled by various factors including microRNAs (miRs) interference, which are small non-coding endogenous RNAs. METHODS: In the current study, the effects of forced miR-148b expression was evaluated on osteogenic activity. Human bone marrow-derived mesenchymal stem cells (BM-MSCs) were transduced with bicistronic lentiviral vector encoding hsa-miR-148b-3p or -5p and the enhanced green fluorescent protein. Fourteen days post-transduction, immunostaining as well as Western blotting were used to analyze osteogenesis. RESULTS: Overexpression of miR-148b-3p increased the osteogenic differentiation of human BM-MSCs as demonstrated by anenhancement of mineralized nodular formation and an increase in the levels of osteoblastic differentiation biomarkers, alkaline phosphatase and collagen type I. CONCLUSIONS: Since lentivirally overexpressed miR-148b-3p increased osteogenic differentiation capability of BM-MSCs, this miR could be applied as a therapeutic modulator to optimize bone function.

Keywords

, Lentivirus; Mesenchymal stem cells; MicroRNA, 148b; Osteogenesis
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@article{paperid:1077683,
author = {سمانه ملازاده and بی بی صدیقه فضلی بزاز and وجیهه نشاطی and Antoine A. F. De Vries and حجت نادری مشکین and مجید مجرد and مهدی میر احمدی and Neshati, Zeinab and Mohammad Amin Kerachian},
title = {Overexpression of MicroRNA-148b-3p stimulates osteogenesis of human bone marrow-derived mesenchymal stem cells: the role of MicroRNA-148b-3p in osteogenesis},
journal = {BMC Medical Genetics},
year = {2019},
volume = {20},
number = {1},
month = {July},
issn = {1471-2350},
keywords = {Lentivirus; Mesenchymal stem cells; MicroRNA-148b; Osteogenesis},
}

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%0 Journal Article
%T Overexpression of MicroRNA-148b-3p stimulates osteogenesis of human bone marrow-derived mesenchymal stem cells: the role of MicroRNA-148b-3p in osteogenesis
%A سمانه ملازاده
%A بی بی صدیقه فضلی بزاز
%A وجیهه نشاطی
%A Antoine A. F. De Vries
%A حجت نادری مشکین
%A مجید مجرد
%A مهدی میر احمدی
%A Neshati, Zeinab
%A Mohammad Amin Kerachian
%J BMC Medical Genetics
%@ 1471-2350
%D 2019

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