Journal of Biological Research - Thessaloniki, Volume (26), No (1), Year (2019-9)

Title : ( T-Box20 inhibits osteogenic differentiation in adipose-derived human mesenchymal stem cells: the role of T-Box20 on osteogenesis )

Authors: Samaneh Mollazadeh , Bibi Sedigheh Fazly Bazzaz , Vajiheh Neshati , Antoine A. F. de Vries , Hojjat Naderi-Meshkin , Majid Mojarad , Zeinab Neshati , Mohammad Amin Kerachian ,

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Abstract

Abstract Background: Skeletal development and its cellular function are regulated by various transcription factors. The T-box (Tbx) family of transcription factors have critical roles in cellular differentiation as well as heart and limbs organogenesis. These factors possess activator and/or repressor domains to modify the expression of target genes. Despite the obvious effects of Tbx20 on heart development, its impact on bone development is still unknown. Methods: To investigate the consequence by forced Tbx20 expression in the osteogenic differentiation of human mesenchymal stem cells derived from adipose tissue (Ad-MSCs), these cells were transduced with a bicistronic lentiviral vector encoding Tbx20 and an enhanced green fluorescent protein. Results: Tbx20 gene delivery system suppressed the osteogenic differentiation of Ad-MSCs, as indicated by reduction in alkaline phosphatase activity and Alizarin Red S staining. Consistently, reverse transcription-polymerase chain reaction analyses showed that Tbx20 gain-of-function reduced the expression levels of osteoblast marker genes in osteo-inductive Ad-MSCs cultures. Accordingly, Tbx20 negatively affected osteogenesis through modulating expression of key factors involved in this process. Conclusion: The present study suggests that Tbx20 could inhibit osteogenic differentiation in adipose-derived human mesenchymal stem cells.

Keywords

, Adipose tissue, derived mesenchymal stem cells; Lentiviral vector; Osteogenesis; Tbx20; Transcription factor
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@article{paperid:1077684,
author = {سمانه مولازاده and بی بی صدیقه فضلی بزاز and وجیهه نشاطی and Antoine A. F. De Vries and حجت نادری مشکین and مجید مجرد and Neshati, Zeinab and محمد امین کراچیان},
title = {T-Box20 inhibits osteogenic differentiation in adipose-derived human mesenchymal stem cells: the role of T-Box20 on osteogenesis},
journal = {Journal of Biological Research - Thessaloniki},
year = {2019},
volume = {26},
number = {1},
month = {September},
issn = {1790-045X},
keywords = {Adipose tissue-derived mesenchymal stem cells; Lentiviral vector; Osteogenesis; Tbx20; Transcription factor},
}

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%0 Journal Article
%T T-Box20 inhibits osteogenic differentiation in adipose-derived human mesenchymal stem cells: the role of T-Box20 on osteogenesis
%A سمانه مولازاده
%A بی بی صدیقه فضلی بزاز
%A وجیهه نشاطی
%A Antoine A. F. De Vries
%A حجت نادری مشکین
%A مجید مجرد
%A Neshati, Zeinab
%A محمد امین کراچیان
%J Journal of Biological Research - Thessaloniki
%@ 1790-045X
%D 2019

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