The present study compares the personality traits between two subcultures of Isfahan and Tehran in different age groups using a causal-comparative assessment. The participants included 661 individuals aged ≥ 20 years old who were selected from the citizenry of Tehran (region 6) and the citizenry of Isfahan (region 3) using stratified random sampling method in five different age groups. To collect data, NEO-FFI and for data analysis independent t-test were used.The results indicated that conscientiousness of the 20-30 years old age group in citizenry of Isfahan was significantly higher than their peers with citizenry of Tehran. Agreeableness in the age group of ≥ 65 years old and neuroticism in the 30-40 years old age group in citizenry of Tehran were significantly higher than the citizenry of Isfahan. The traits of extroversion and openness to experiences were significantly different between citizenry of two subcultures. Further studies on cultural psychology and on other subcultures seem necessary.

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