Title : ( Characterizing the Binding of Angiotensin Converting Enzyme I Inhibitory Peptide to Human Hemoglobin: Influence of Electromagnetic Fields )
Authors: Farzaneh Sadeghzadeh , Amir Arsalan Entezari , Kiana Behzadian , Kimia Habibi , Zeinab Amiri-Tehranizadeh , Ahmad Asoodeh , Mohammad Reza Saberi , Jamshidkhan Chamani ,
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Abstract
Background: Drug-protein complexes is one of the crucial factors when analyzing the pharmacokinetics and pharmacodynamics of a drug because they can affect the excretion, distribution, metabolism and interaction with target tissues. Objectives: The aim of this study was to investigate the interaction of human hemoglobin (Hb) and angiotensin I converting enzyme inhibitory peptide (ACEIP) in the absence and presence of different- frequency electromagnetic fields (EMF). Methods: Various spectroscopic methods like fluorescence spectroscopy, ultraviolet, circular dichroism and conductometry techniques were applied to investigate Hb-ACEIP interaction in the absence and presence of EMF Result: The presented spectroscopic studies indicated that EMF changed the interaction between Hb and ACEIP. The a-helix content of Hb decreased upon binding to ACEIP and conductivity of the solution enhanced upon binding. Based on Stern-Volmer equations, it could be stated that the Hb-ACEIP affinity was higher in the presence of EMF. Conclusion: It can be concluded that for patients who use the drug to control blood pressure, a low-frequency electromagnetic field would have a positive effect on the uptake of the drug.
Keywords
, Drug-protein complexes, ACE inhibitory peptide, human hemoglobin, electromagnetic field, fluorescence spectroscopy, circular dichroism.
@article{paperid:1080705,
author = {Farzaneh Sadeghzadeh and Amir Arsalan Entezari and Kiana Behzadian and Kimia Habibi and Zeinab Amiri-Tehranizadeh and Asoodeh, Ahmad and Mohammad Reza Saberi and Jamshidkhan Chamani},
title = {Characterizing the Binding of Angiotensin Converting Enzyme I Inhibitory Peptide to Human Hemoglobin: Influence of Electromagnetic Fields},
journal = {Protein and Peptide Letters},
year = {2020},
volume = {27},
number = {10},
month = {April},
issn = {0929-8665},
pages = {1007--1021},
numpages = {14},
keywords = {Drug-protein complexes; ACE inhibitory peptide; human hemoglobin; electromagnetic field; fluorescence spectroscopy;
circular dichroism.},
}
%0 Journal Article
%T Characterizing the Binding of Angiotensin Converting Enzyme I Inhibitory Peptide to Human Hemoglobin: Influence of Electromagnetic Fields
%A Farzaneh Sadeghzadeh
%A Amir Arsalan Entezari
%A Kiana Behzadian
%A Kimia Habibi
%A Zeinab Amiri-Tehranizadeh
%A Asoodeh, Ahmad
%A Mohammad Reza Saberi
%A Jamshidkhan Chamani
%J Protein and Peptide Letters
%@ 0929-8665
%D 2020
