Current Microbiology, ( ISI ), Volume (77), No (7), Year (2020-7) , Pages (1254-1261)

Title : ( Purification and Biochemical Characterization of Alkalophilic Cellulase from the Symbiotic Bacillus subtilis BC1 of the Leopard Moth, Zeuzera pyrina (L.) (Lepidoptera: Cossidae) )

Authors: Fahimeh Dehghanikhah , Jahanshir Shakarami , Ahmad Asoodeh ,

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Abstract

In the current study, an extracellular cellulase belonging to symbiotic Bacillus subtilis Bc1 of the leopard moth is purified and characterized. The molecular mass of enzyme was 47.8 kDa using SDS-PAGE. The purified enzyme had optimum activity in temperature and pH around 60 °C and 8, respectively. The purified cellulase was introduced as a stable enzyme in a wide variety of temperature (20–80 °C) and pH (4–10) and remained active to more than 74% at 80 °C for 1 h. Moreover, the cel-lulase extremely was stabled in the presence of metal ions and organic solvents and its activity was increased by acetone (20% v/v), CaCl2 and CoCl2 and inhibited by MnCl2 and NiCl2. The values of enzyme’s Km and Vmax were found to be 1.243 mg/mL and 271.3 μg/mL/min, respectively. The purified cellulase hydrolyzed cellulose, avicel and carboxymethyl cellulose (CMC) and the final product of CMC hydrolysis was cellobiose using thin-layer chromatography analysis. Consequently, owing to exo/endoglucanase activity and organic solvent, temperature and pH stability of the purified cellulase belong to B. subtilis BC1, it can be properly employed for various industrial purposes.

Keywords

Cellulase · Zeuzera pyrina · Cellulolytic bacteria · Bacillus subtilis
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@article{paperid:1080706,
author = {فهیمه دهقانی خواه and جهانشیر شاکرمی and Asoodeh, Ahmad},
title = {Purification and Biochemical Characterization of Alkalophilic Cellulase from the Symbiotic Bacillus subtilis BC1 of the Leopard Moth, Zeuzera pyrina (L.) (Lepidoptera: Cossidae)},
journal = {Current Microbiology},
year = {2020},
volume = {77},
number = {7},
month = {July},
issn = {0343-8651},
pages = {1254--1261},
numpages = {7},
keywords = {Cellulase · Zeuzera pyrina · Cellulolytic bacteria · Bacillus subtilis},
}

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%0 Journal Article
%T Purification and Biochemical Characterization of Alkalophilic Cellulase from the Symbiotic Bacillus subtilis BC1 of the Leopard Moth, Zeuzera pyrina (L.) (Lepidoptera: Cossidae)
%A فهیمه دهقانی خواه
%A جهانشیر شاکرمی
%A Asoodeh, Ahmad
%J Current Microbiology
%@ 0343-8651
%D 2020

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