Mannoprotein (mannan) extracted from the cell wall of Saccharomyces cerevisiae is a bioactive glycoprotein that has many biological effects. In the present work, the preparation of gelatinbased blend films containing different concentrations of mannan (0, 0.5, 1.0, and 1.5%) was investigated. The effects of mannan on moisture content, water solubility, water vapor permeability (WVP), mechanical, optical, and thermal features of films were investigated. Moreover, the antifungal and aflatoxin B1 (AFB1) binding traits of the developed films were also studied. The results indicated that the addition of mannan significantly increased the water solubility, elongation at break, and WVP, whereas glass transition temperature and elastic modulus drastically decreased in the blend films. Furthermore, an obvious interaction between mannan and gelatin was concluded from Fourier-Transform Infrared (FTIR) spectra. Scanning electron microscopy assessment of the films revealed a heterogeneous structure. According to antifungal assay, the gelatin-mannan blend films had antifungal effect against Aspergillus flavus in disc diffusion evaluation. Similarly, these active films could reduce AFB1 content of pistachio extract agar.

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