ASN Neuro, Volume (8), No (6), Year (2016-12) , Pages (2147483647-41667500)

Title : ( Assessment of Neuroprotective Properties of Melissa officinalis in Combination With Human Umbilical Cord Blood Stem Cells After Spinal Cord Injury )

Authors: Seyed Ruhollah Hosseini , Gholamreza Kaka , MohammadTaghi Joghataei , Mehdi Hooshmandi , Seyed Homayoon Sadraie , Kayvan Yaghoobi , Alireza Mohammadi ,

Citation: BibTeX | EndNote

Abstract

Introduction: The pathophysiology of spinal cord injury (SCI) has a classically bad prognosis. It has been demonstrated that human umbilical cord blood stem cells (hUCBSCs) and Melissa officinalis (MO) are useful for the prevention of neurological disease. Methods: Thirty-six adult male rats were randomly divided into intact, sham, control (SCI), MO, hUCBSC, and MO-hUCBSC groups. Intraperitoneal injection of MO (150 mg/kg) was commenced 24 hr post-SCI and continued once a day for 14 days. Intraspinal grafting of hUCBSCs was commenced immediately in the next day. The motor and sensory functions of all animals were evaluated once a week after the commencement of SCI. Electromyography (EMG) was performed in the last day in order to measure the recruitment index. Immunohistochemistry, reverse transcription-polymerase chain reaction, and transmission electron microscopy evaluations were performed to determine the level of astrogliosis and myelination. Results: The results revealed that motor function (MO-hUCBSC: 15 0.3, SCI: 8.2 0.37, p <.001), sensory function (MOhUCBSC: 3.57 0.19, SCI: 6.38 0.23, p <.001), and EMG recruitment index (MO-hUCBSC: 3.71 0.18, SCI: 1.6 0.1, p <.001) were significantly improved in the MO-hUCBSC group compared with SCI group. Mean cavity area (MO-hUCBSC: 0.03 0.03, SCI: 0.07 0.004, p <.001) was reduced and loss of lower motor neurons (MO-hUCBSC: 7.6 0.43, SCI: 3 0.12, p <.001) and astrogliosis density (MO-hUCBSC: 3.1 0.15, SCI: 6.25 1.42, p < 0.001) in the ventral horn of spinal cord were prevented in MO-hUCBSC group compared with SCI group. Conclusion: The results revealed that the combination of MO and hUCBSCs in comparison with the control group has neuroprotective effects in SCI.

Keywords

, astrogliosis, human umbilical cord blood, Melissa officinalis, myelination, spinal cord injury
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@article{paperid:1081168,
author = {Hosseini, Seyed Ruhollah and Gholamreza Kaka and MohammadTaghi Joghataei and Mehdi Hooshmandi and Seyed Homayoon Sadraie and Kayvan Yaghoobi and Alireza Mohammadi},
title = {Assessment of Neuroprotective Properties of Melissa officinalis in Combination With Human Umbilical Cord Blood Stem Cells After Spinal Cord Injury},
journal = {ASN Neuro},
year = {2016},
volume = {8},
number = {6},
month = {December},
issn = {1759-0914},
pages = {2147483647--41667500},
numpages = {-2105816147},
keywords = {astrogliosis; human umbilical cord blood; Melissa officinalis; myelination; spinal cord injury},
}

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%0 Journal Article
%T Assessment of Neuroprotective Properties of Melissa officinalis in Combination With Human Umbilical Cord Blood Stem Cells After Spinal Cord Injury
%A Hosseini, Seyed Ruhollah
%A Gholamreza Kaka
%A MohammadTaghi Joghataei
%A Mehdi Hooshmandi
%A Seyed Homayoon Sadraie
%A Kayvan Yaghoobi
%A Alireza Mohammadi
%J ASN Neuro
%@ 1759-0914
%D 2016

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