Journal of Cell and Molecular Research, Volume (11), No (2), Year (2020-3) , Pages (55-58)

Title : ( A Rapid Method for Analysis of cDNA Synthesis Using Ion-Pair Reversed-Phase High Performance Liquid Chromatography )

Authors: Maryam Moghaddam Matin , David P. Hornby ,

Citation: BibTeX | EndNote

Abstract

We have developed a rapid, quantitative method for analysing the outcome of the first strand synthesis step in cDNA library preparation, yield and molecular weight range of the final cDNA products are determined after size fractionation. This method involves conventional cDNA library construction including all enzymatic steps usually required, but replaces radioactive labelling of nucleic acids with fluorescence detection. The separation and quantification steps all involve ion-pair reversed-phase high performance liquid chromatography (IP RP HPLC). This quantitative method replaces the use of autoradiography and size exclusion chromatography with combined ion-pair reversed-phase high performance liquid chromatography and in line fluorescence detection. The result of this approach is combination of speed with the generation of reproducible, high quality cDNA libraries.

Keywords

, cDNA, library construction, IP RP HPLC, size fractionation
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@article{paperid:1081829,
author = {Moghaddam Matin, Maryam and David P. Hornby},
title = {A Rapid Method for Analysis of cDNA Synthesis Using Ion-Pair Reversed-Phase High Performance Liquid Chromatography},
journal = {Journal of Cell and Molecular Research},
year = {2020},
volume = {11},
number = {2},
month = {March},
issn = {2008-9147},
pages = {55--58},
numpages = {3},
keywords = {cDNA; library construction; IP RP HPLC; size fractionation},
}

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%0 Journal Article
%T A Rapid Method for Analysis of cDNA Synthesis Using Ion-Pair Reversed-Phase High Performance Liquid Chromatography
%A Moghaddam Matin, Maryam
%A David P. Hornby
%J Journal of Cell and Molecular Research
%@ 2008-9147
%D 2020

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