Background: Oxidative stress is a significant problem for centuries and the origin of various diseases from cataracts to cancer. Free radicals are atoms or molecules with a single electron that is remarkably reactive and can be irreversible damage to vital molecules in organisms. Antioxidants are capable to protect biological systems against these elements. Iron nanoparticle (Fe-NPs) has antimicrobial, anticancer, and antioxidant properties. This paper describes the antioxidant activities of green synthesis Fe-NPs. Methods: The nanoparticle of Fe-NPs was synthesis through employing the aqueous leaves extract of Saponaria officinalis. Antioxidant activity of iron nanoparticles in the range of 0-250 μg/ml was measured using 2,2-diphenyl- 1-picrylhydrazyl (DPPH) radical scavenging assay. Ascorbic acid (vitamin C) was considered for positive control. Finally, the absorption of samples was measured at 517 nm. Results: Antioxidant activity of Fe3O4 nanoparticles was measured by DPPH. The DPPH radical scavenging effect of 250 μg/mL Fe3O4-NPs was about 56.25 %, while standard samples at the same concentration showed 87.5 % radical scavenging effect. By increasing the concentration of nanoparticles, the percentage of radical scavenging increased. Conclusion: The present study demonstrated that the aquatic extract of Saponaria officinalis has a natural antioxidant source and might be exploited for functional foods and nutraceutical applications.

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