Increasing iron (Fe) concentration in food crops is a global challenge thatdepend on the type and methods of fertilizer application in agriculturepractices. Fe-organic complexes (Fe-OCs) including Fe-Glycine (Fe-Gly), Fe-Tyrosine (Fe-Try), and Fe-Chitosan (Fe-Chi) were synthesized and character-ized by different analytical techniques. A greenhouse experiment was con-ducted to evaluate the role of soil, fertigation, and the foliar application ofFe-OCs in comparison to Fe-EDDHA to improve Fe status in wheat(Triticum aestivum,cv. N91-8). The finding of elemental analysis supportedthe formation of Fe-OCs. The soil application of Fe-OCs increased the yieldand yield components of the wheat. Further, the shoot Fe concentrationand other macronutrients increased due to the Fe-OCs with the soil appli-cation, however, Fe-Gly and Fe-Tyr more than Fe-Chi resulted in anincrease in Fe concentration. Fe-OCs improved nitrogen (N) status in theshoot and grain, and Fe in the grain. The foliar application of Fe-Gly, Fe-Tyr, and Fe-Chi increased Fe concentration in the grain compared to Fe-EDDHA. Fe-OCs can serve as an important agronomic practice to improveFe grain concentration

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