Bromophenol blue (BPB)-doped nano-droplets are recognized as a synthesis of BPB dye and transparent dispersion of nano-droplets (ND), in a continuous phase of oils. In this regard, to discover the effect of ND size on nonlinear optical properties of BPB, a solution of BPB-doped ND with heptane oil and sodium bis(2-ethylhexyl) sulfosuccinate (AOT) as a surfactant has been prepared and therefore investigated with Z-scan technique. The consequences have revealed that the nonlinear refractive (NLR) index, of the BPB-doped ND has been improved by comparison with BPB aqueous solution. The second-order hyperpolarizability of the BPB-doped ND has been also reduced subsequent a reduction in ND size owing to reducing dye aggregation. Moreover, the BPB-doped ND in the company of laser radiation has been utilized in order to study of antibacterial photodynamic therapy of Staphylococcus aureus (S. aureus) by nonlinear absorption. As a consequence, the bacteria colonies have been also exceedingly declined in the group treated with the BPB-doped ND.

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