Various methods have been used to treat industrial effluents from dyes. In this study, the removal of two xanthene dyes usinglaccase enzyme was investigated. Lacases are oxidizing enzymes and have been studied in many studies due to their wide range of substrates. One of the capabilities of laccase is the bleaching of synthetic dyes. In this study, the removal of two dyes, erythrosine B and rhodamine 6G, which are members of the xanthene group, as well as neutral red dye by spore laccase from Bacillus sp KC2 in approximately 24 hours using spectrophotometry, Spectrofluorometry and Z-scan methods were studied. The results of Z-scan showed removal of erythrosine B from aqueous solvent by 45%, rhodamine 6G by 49% and neutral red by 68%

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