Mastitis is one of the most significant diseases resulting in an inflammatory reaction in the mammary gland of dairy cattle. Administration of antimicrobial drugs for treatment leads to drug-resistance bacteria or fungi. Hence, the use of natural alternatives such as antimicrobial peptides and plant extracts has been considered as alternatives to common antibiotics. The aim of this study was to introduce natural compounds with suitable antifungal activity as an alternative to chemical methods. The minimum inhibitory concentration (MIC) and the minimum fungicidal concentration (MFC) were determined based on broth microdilution method by 96-well plate with four replications using chinaberry (Melia azedarach) extract and thanatin peptide on three mastitis-causing fungi. The results showed that the thanatin was effective on growth inhibition of all three fungi. Thanatin is more effective against Candida albicans and parapsilosis. Chinaberry extract with MIC of 6250 µg / ml and MFC of 12500 µg / ml had the greatest effect on Candida parapsilosis. Applied chinaberry extract had no effect on Mucor spp fungi while thanatin showed an antifungal activity against this species. This study showed that compounds of plant extracts and peptides had beneficial antifungal activities. It is promising that these compounds could be used as alternatives to assist treat infectious diseases such as mastitis.

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