4th International and 16th Iranian Genetics Congress , 2020-09-30

Title : ( Expression and function of C1orf132 long-noncoding RNA, in breast cancer cell lines and tissues )

Authors: Afsaneh Malekzadeh Shafaroudi , Ali Sharifi zarchi , Nahid Nafisi , Salvatore Oliviero , Maryam Moghaddam Matin ,

Citation: BibTeX | EndNote

Abstract

Background and Aim: miR-29b2 and miR-29c play a suppressive role in breast cancer progression. Mir29b2chg/C1orf132 is the host gene for generating both microRNAs. However, there existed longer transcripts of the gene, with unknown function. Methods: Here, we have employed bioinformatics and experimental approaches to decipher expression of two different C1orf132 transcripts in different types of breast cancer cell lines and tissues. Results: Our data demonstrated a significant downregulation of C1orf132 in triple-negative type of breast cancer. We also find out that a mouse homolog of C1orf132 is located at a similar genomic location as its human counterpart, between CD46 and CD34 genes. Conclusion: The latter finding suggests new potential functions for the lncRNA, other than being a simple miR-29 host gene. We predicted a putative promoter for the longer transcripts of C1orf132. The functionality of the distal promoter confirmed by transfecting MCF7 cells with a C1orf132 promoter-GFP construct. Knocking-down the promoter by means of the Crispr/Cas9 approach revealed no alteration in the expression level of neighboring genes, CD46 and CD34. However, the expression level of miR-29c was reduced by half, suggesting an enhancer effect of the distal promoter on miR-29cf generation. Furthermore, the promoter knock-down revealed a G2/M cell cycle arrest and an elevation of migration ability in Mcf12a edited cells. Moreover, the expression of cell mobility genes e.g. CDH2, FGF2, FGFR1 and the stem cell and EMT -associated transcription factor ZEB1 was significantly overexpressed in edited cells. Altogether, we are reporting here the existence of an additional/distal promoter with an enhancer effect on miR-29 generation and an inhibitory effect on cell proliferation and cell migration.

Keywords

, Long-noncoding RNA, Triple-negative breast cancer, Crispr/Cas9, EMT
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@inproceedings{paperid:1084477,
author = {Malekzadeh Shafaroudi, Afsaneh and علی شریفی زارچی and ناهید نفیسی and Salvatore Oliviero and Moghaddam Matin, Maryam},
title = {Expression and function of C1orf132 long-noncoding RNA, in breast cancer cell lines and tissues},
booktitle = {4th International and 16th Iranian Genetics Congress},
year = {2020},
location = {تهران, IRAN},
keywords = {Long-noncoding RNA; Triple-negative breast cancer; Crispr/Cas9; EMT},
}

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%0 Conference Proceedings
%T Expression and function of C1orf132 long-noncoding RNA, in breast cancer cell lines and tissues
%A Malekzadeh Shafaroudi, Afsaneh
%A علی شریفی زارچی
%A ناهید نفیسی
%A Salvatore Oliviero
%A Moghaddam Matin, Maryam
%J 4th International and 16th Iranian Genetics Congress
%D 2020

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