Title : ( A novel organic-solvent and detergent resistant esterase from Bacillus sp. isolated from Bazangan Lake )
Authors: Fatemeh Pasban Ziyarat , Jamshid Mehrzad , Ahmad Asoodeh , behjat deiminiat , Alireza Motavalizadehkakhky ,
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Abstract
This study reports biochemical features of a novel esterase of Bacillus sp. HP 96 isolated from Bazangan Lake in Iran. The optimum pH and temperature for 53.4 kDa-purified esterase were 8 and 40 C, respectively. The HPLC technique was used to examine the main compound produced by the enzyme on nitrophenyl derivatives. Vmax and Km of esterase towards 4-nitrophenyl butyrate (p-NPB) were 75.12 mmol.min1mg1 and 4.66mM, respectively. Organic solvents (15, 30% v/v) did not have a significant effect on the stability of the purified enzyme. The Bacillus sp. HP 96 esterase showed a high compatibility towards various ionic liquids and detergents. In conclusion, the characterized esterase has potential to be used in detergent formularizations and biotechnological processes under harsh conditions.
Keywords
, Esterase; HPLC; 4, nitrophenyl butyrate; detergent; organic solvent
@article{paperid:1084573,
author = {Fatemeh Pasban Ziyarat and Jamshid Mehrzad and Asoodeh, Ahmad and Deiminiat, Behjat and Alireza Motavalizadehkakhky},
title = {A novel organic-solvent and detergent resistant esterase from Bacillus sp. isolated from Bazangan Lake},
journal = {Biocatalysis and Biotransformation},
year = {2021},
volume = {1},
number = {1},
month = {May},
issn = {1024-2422},
pages = {1--12},
numpages = {11},
keywords = {Esterase; HPLC; 4-
nitrophenyl butyrate;
detergent; organic solvent},
}
%0 Journal Article
%T A novel organic-solvent and detergent resistant esterase from Bacillus sp. isolated from Bazangan Lake
%A Fatemeh Pasban Ziyarat
%A Jamshid Mehrzad
%A Asoodeh, Ahmad
%A Deiminiat, Behjat
%A Alireza Motavalizadehkakhky
%J Biocatalysis and Biotransformation
%@ 1024-2422
%D 2021
