Journal of Cell and Molecular Research, Year (2021-7)

Title : ( Royal Jelly Decreases MMP-9 Expression and Induces Apoptosis in Human Bladder Cancer 5637 Cells )

Authors: narges fazili , Zahra Soheila Soheili , Saeid Malekzadeh Shafaroudi , Shahram Samiei , Shamila D. Alipoor , Nasrin Moshtaghi , Abouzar Bagheri ,

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Abstract

Royal jelly (RJ) as a traditional medicinal agent has a variety of pharmacological benefits. In the present study, the effects of the royal jelly were investigated on the urinary bladder cancer cell line 5637 (HTB-9). MTT assay was performed to determine the percent of cell viability at different concentrations of the royal jelly. Moreover, in vitro wound-healing assay was applied to investigate the effects of RJ on cell migration. The activities and gene expression levels of matrix metalloproteinase 2 and 9 were assessed by zymography and Real time PCR, respectively. It was confirmed that royal jelly (RJ) at the concentration of 0.7 mg/ml exerted a significant inhibitory effect on HTB 5637 cells and reduced cell viability to 72% in comparison to the control cultures (P-value<0.009) during the first 72h of treatment. Furthermore, royal jelly Significantly decreased the bladder cancerous cell migration capacity, and induced a significant decrease in the transcriptional level of the MMP9 after 72h (50% of the controls; P-value<0.049). However, R.J.S did not impose any effect on the expression level and activity of matrix metalloproteinase 2. The results indicated the potential of RJ as a promising natural anti-proliferative and anti-metastatic drug.

Keywords

, Bladder Cancer, Royal jelly, MMP-9, MMP2
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@article{paperid:1086647,
author = {Fazili, Narges and Zahra Soheila Soheili and Malekzadeh Shafaroudi, Saeid and Shahram Samiei and Shamila D. Alipoor and Moshtaghi, Nasrin and Abouzar Bagheri},
title = {Royal Jelly Decreases MMP-9 Expression and Induces Apoptosis in Human Bladder Cancer 5637 Cells},
journal = {Journal of Cell and Molecular Research},
year = {2021},
month = {July},
issn = {2008-9147},
keywords = {Bladder Cancer; Royal jelly; MMP-9; MMP2},
}

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%0 Journal Article
%T Royal Jelly Decreases MMP-9 Expression and Induces Apoptosis in Human Bladder Cancer 5637 Cells
%A Fazili, Narges
%A Zahra Soheila Soheili
%A Malekzadeh Shafaroudi, Saeid
%A Shahram Samiei
%A Shamila D. Alipoor
%A Moshtaghi, Nasrin
%A Abouzar Bagheri
%J Journal of Cell and Molecular Research
%@ 2008-9147
%D 2021

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