Gastric and colon carcinomas are among the most prevalent cancers with high mortality rates worldwide, and therefore, introduction of new anticancer agents has been widely considered for their treatment. The aim of present study was to determine cytotoxicity of exopolysaccharide (EPS) derived from a Vibrio species on human gastric and colon cancer cells. To do so, after isolation of EPS from a Vibrio strain VB23, MKN45 and LoVo cells were treated with 25, 50, 100 and 200 μg/ml of VB23 EPS. Upon 24 and 48 h, cell viability was evaluated by resazurin as a colorimetric assay. Results of viability assay indicated that 200 μg/ml of VB23 EPS induced highest toxic effects on MMKN45 cells after 24 h. However, 100 μg/ml VB23 EPS induced highest cytotoxicity on LoVo cells after 24 h. Accordingly, obtained results revealed that cytotoxic effects of VB23 EPS were time- and cell type-dependent.

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