The present study was conducted to develop a simple, low-cost and sensitive micro fluidic paper-based analytical device (µPAD) for analysis of gold in geological samples. This µPAD was fabricated employing eyeliner pencil method. It consists of different zones, including detection, pH adjustment, reaction and elution. We injected 29 µL elution solution in the center of µPAD, direct Au3+, after adjusting pH, to make it react with rhodamine B. The formation of RB-Au3+ complex led to quench of rhodamine B fluorescence. The image of each detection zone was captured using a cell phone camera under a UV lamp, and luminosity intensity was subsequently measured with Photoshop software. Various parameters were studied in order to achieve the most efficient performance of the paper sensor. Under the optimized condition, a wide linear range (1–40 mg L−1, R2 = 0.996) was obtained with detection limit of 0.15 mg L−1. Ultimately, the developed µPAD was applied for the determination of gold in ore samples. The obtained results were in good agreement with those obtained from standard method. The combination of fire assay and µPAD in the determination of gold in geological samples improved the detection limit based on enrichment factor of 75.

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