Background: Colorectal cancer is one of the leading cause of cancer-associated death in both men and women, all around the world. Therefor molecular target therapy can play a key role in CRC treatment approach. Next generation sequencing technologies promises new insight into the dynamic of transcription of cancer. This work will broaden our knowledge of the molecular dynamic of mRNAs at transcription level and allow the identification of recurrent fusion genes in colorectal cancer. Methods: This project has been carried out using RNA-seq data from NCBI data bank (PRJEB27536 bioproject). A total of 60 matched samples were analyzed with FusionCatcher V.1.20. A list of fusions were constructed for all the cancer samples. Finally the frequency of recurrent fusions were evaluated. Results: REG4, CEACAM5 and OLFM4 are the most recurrently fused genes present in cancer samples in comparison with normal samples. REG4 is a very hyperactive mRNA in terms transcriptional dynamics and is involved in the most frequent fusions in cancer such as PIGR-REG4, MUC2-REG4 and KRT8-REG4. Other recurrent fusions are CEACAM5-MUC2, CEACAM5-KRT8 and OLFM4-PIGR. Conclusion: Our study revealed that REG4, CEACAM5 and OLFM4 are amongst the most frequent fusions found in our cancer data. The oncogenic role of these fusions can be investigated with further functional analysis. Some of these fusions can be druggable and may be a good candidate for molecularly targeted therapy.

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