The Gobiidae is one of the largest families of fish in the world. This study deals with the morphometric characteristics as well as the geometric morphology of the specimens belong to three species from Gobiidae family, i.e. Acentrogobius dayi Koumans, 1941, Bathygobius meggitti (Hora and Mukerji, 1936) and Istigobius ornatus (Rüppell, 1830) from Makran coast, South-East of Iran. A total of 56 specimens were captured from the three stations Tis, Lipar and Darya-Bozorg, from spring to summer 2018. 36 morphological variables were analysed (including univariate and multivariate), using SPSS and PAST software. Also, to test the morphological plasticity of the specimens, the dorsal, lateral (left side) and ventral sides of the specimens were photographed. 12 Landmarks from the dorsal side, 20 Landmarks from the latral side and 19 Landmarks from the ventral side of the specimens were digitized using tpsDig software. The shape data, based on landmarks coordinates, data were investigated using multivariate analyses. Body shape of each population was visualized based on the average total shape. The results revealed a significant difference in body shape between the studied species. Based on a cluster analysis, A.dayi and I.ornatus species were more similar and most difference was observed between B.meggitti and other species. The observed morphological differences could be relevant to the environmental conditions and feeding habits of the studied species. To what degree this continuous data can now be translated into discrete and diagnostic features, useful for taxonomic purposes, remains to be studied.

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