Frontiers in Bioengineering and Biotechnology, Volume (10), Year (2023-1)

Title : ( Using magnetic mesoporous silica nanoparticles armed with EpCAM aptamer as an efficient platform for specific delivery of 5-fluorouracil to colorectal cancer cells )

Authors: ASEEL MOHAMMED , Ahmad Reza Bahrami , Sirous Nekooei , Amir SHokooh Saljooghi , Maryam Moghaddam Matin ,

Citation: BibTeX | EndNote

Abstract

Background: Theranostic nanoparticles with both imaging and therapeutic capacities are highly promising in successful diagnosis and treatment of advanced cancers. Methods: Here, we developed magnetic mesoporous silica nanoparticles (MSNs) loaded with 5-fluorouracil (5-FU) and surface-decorated with polyethylene glycol (PEG), and epithelial cell adhesion molecule (EpCAM) aptamer (Apt) for controlled release of 5-FU and targeted treatment of colorectal cancer (CRC) both in vitro and in vivo. In this system, Au NPs are conjugated onto the exterior surface of MSNs as a gatekeeper for intelligent release of the anti-cancer drug at acidic conditions. Results: Nanocarriers were prepared with a final size diameter of 78 nm, the surface area and pore size of SPION-MSNs were calculated as 636 m 2 g−1 , and 3 nm based on the BET analysis. The release of 5-FU from nanocarriers was pH-dependent, with an initial rapid release (within 6 h) followed by a sustained release for 96 h at pH 5.4. Tracking the cellular uptake by flow cytometry technique illustrated more efficient and higher uptake of targeted nanocarriers in HT-29 cells compared with non-targeted formula. In vitro results demonstrated that nanocarriers inhibited the growth of cancer cells via apoptosis induction. Furthermore, the targeted NPs could significantly reduce tumor growth in immunocompromised C57BL/6 mice bearing HT-29 tumors, similar to those injected with free 5-FU, while inducing less side effects. Conclusion: These findings suggest that application of Apt-PEG-Au-NPs@5-FU represents a promising theranostic platform for EpCAM-positive CRC cells, although further experiments are required before it can be practiced in the clinic.

Keywords

, colorectal cancer, mesoporous silica nanoparticles, superparamagnetic iron oxide, 5- fluorouracil, epithelial cell adhesion molecule
برای دانلود از شناسه و رمز عبور پرتال پویا استفاده کنید.

@article{paperid:1092920,
author = {MOHAMMED, ASEEL and Bahrami, Ahmad Reza and Sirous Nekooei and SHokooh Saljooghi, Amir and Moghaddam Matin, Maryam},
title = {Using magnetic mesoporous silica nanoparticles armed with EpCAM aptamer as an efficient platform for specific delivery of 5-fluorouracil to colorectal cancer cells},
journal = {Frontiers in Bioengineering and Biotechnology},
year = {2023},
volume = {10},
month = {January},
issn = {2296-4185},
keywords = {colorectal cancer; mesoporous silica nanoparticles; superparamagnetic iron oxide; 5- fluorouracil; epithelial cell adhesion molecule},
}

[Download]

%0 Journal Article
%T Using magnetic mesoporous silica nanoparticles armed with EpCAM aptamer as an efficient platform for specific delivery of 5-fluorouracil to colorectal cancer cells
%A MOHAMMED, ASEEL
%A Bahrami, Ahmad Reza
%A Sirous Nekooei
%A SHokooh Saljooghi, Amir
%A Moghaddam Matin, Maryam
%J Frontiers in Bioengineering and Biotechnology
%@ 2296-4185
%D 2023

[Download]