This work aims to investigate the effect of fuel ratio on the synthesis of CuO nanoparticles via solution combustion and evaluation of its antibacterial properties. Hexamethylenetetramine was considered as fuel and used in different ratio. The final products were characterized by XRD, DLS, FE-SEM analysis. The XRD results indicated that the highest quantity of CuO was found in fuel ratio equal to1. Based on particle size analysis results, all particles were in the range of 21.7 to 42.2 nms. Also, the finest particle size (21.7nm) was attributed to the sample in fuel ratio equal to 1. Since the FE-SEM image of this specimen showed a spherical fine morphology, this specimen was chosen for antibacterial tests. In order to trace the second aim of this study, chosen specimen was tested against Escherichia coli and Staphylococcus aureus bacterial strains while suspended in nutrient broth, water and DMSO. The results revealed higher inhibition of E. coli (gram-negative strain) comparing to S. aureus (gram-positive strain) in all cases. Also, the CuO-nutrient broth appeared to be the suspension with the highest inhibitory for both bacterial strains. The Minimum inhibitory concentration of nanoparticles in this suspension are 105 and 242 µg/mL for E. coli and S. aureus. This could be related to the better dispersion of nanoparticles in nutrient broth solution. Therefore, it could be suggested that adding surfactants to suspension, can enhance the inhibition of bacterial strains.

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