Melanoma is one of the most common types of cancer globally. Probiotics are nonpathogenic bacteria with valuable pharmaceutical and biological activities. The anti-tumor effects of probiotic strains of Lactobacillus have been indicated in various studies. The aim of current research was to investigate the effect of Lactobacillus rhamnosus on the migration of murine melanoma cells. To do so, B16F10 melanoma cells were seeded in 24-well plates, and after 24 hour incubation, a straight scratch was created on the cell monolayer. Then, cells were treated with the supernatant of heat-killed L. rhamnosus (dilution 1:2.5 with complete medium) and control cells were treated with the same dilution of phosphate buffer saline. Photomicrographs were taken from the scratch area at 0 and 24 h, and images were analyzed by ImageJ software. Our results demonstrated that L. rhamnosus reduced the migration ability of B16F10 cells in comparison with control treatment. According to obtained results, L. rhamnosus could be considered as an effective probiotic to inhibit migration of melanoma cells.

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