Title : ( Application of High-Throughput Sequencing for Comprehensive Virome Profiling in Grapevines Shows Yellows in Iran )
Authors: zahra gholampour , Mohammad Zaki AGhl , Elisa Asquini , Mirko Moser , Valeria Gualandri , Mohsen Mehrvar , Azeddine Si-Ammour ,
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Abstract
A comprehensive study on the whole spectrum of viruses and viroids in five Iranian grapevine cultivars was carried out using sRNA libraries prepared from phloem tissue. A comparison of two approaches to virus detection from sRNAome data indicated a significant difference in the results and performance of the aligners in viral genome reconstruction. The results showed a complex virome in terms of viral composition, abundance, and richness. Thirteen viruses and viroids were identified in five Iranian grapevine cultivars, among which the grapevine red blotch virus and grapevine satellite virus were detected for the first time in Iranian vineyards. Grapevine leafrollassociated virus 1 (GLRaV1) and grapevine fanleaf virus (GFLV) were highly dominant in the virome. However, their frequency and abundance were somewhat different among grapevine cultivars. The results revealed a mixed infection of GLRaV1/grapevine yellow speckle viroid 1 (GYSVd1) and GFLV/GYSVd1 in grapevines that exhibited yellows and vein banding. We also propose a threshold of 14% of complete reconstruction as an appropriate threshold for detection of grapevine viruses that can be used as indicators for reliable grapevine virome profiling or in quarantine stations and certification programs.
Keywords
virome; sRNAome; virus mixed infection; Vitis vinifera; Iran
@article{paperid:1098218,
author = {Gholampour, Zahra and Zaki AGhl, Mohammad and Elisa Asquini and Mirko Moser and Valeria Gualandri and Mehrvar, Mohsen and Azeddine Si-Ammour},
title = {Application of High-Throughput Sequencing for Comprehensive Virome Profiling in Grapevines Shows Yellows in Iran},
journal = {Viruses},
year = {2024},
volume = {16},
number = {2},
month = {January},
issn = {1999-4915},
pages = {204--223},
numpages = {19},
keywords = {virome; sRNAome; virus mixed infection; Vitis vinifera; Iran},
}
%0 Journal Article
%T Application of High-Throughput Sequencing for Comprehensive Virome Profiling in Grapevines Shows Yellows in Iran
%A Gholampour, Zahra
%A Zaki AGhl, Mohammad
%A Elisa Asquini
%A Mirko Moser
%A Valeria Gualandri
%A Mehrvar, Mohsen
%A Azeddine Si-Ammour
%J Viruses
%@ 1999-4915
%D 2024
