Hydatidosis is one of the zoonoses, which is very important in the field of medicine, veterinary and also economy. The adult echinococcus granulosus, which lives in the small intestine of canines is swallowed by intermediate host (human and sheep). This parasite causes cyst in tissues, which called ”hydatid cyst”. Hydatid cyst contains hydatid fluid (HF) and also hydatid sand. These proteins stimulate immune system and produce antibody in host body. This research was done on ten samples of hydatid fluid of sheep and one from human. Hydatid fluids were separated from sand hydatid by centrifugation in order to determine dominant subunits HF, similarities and differences between human and sheep. HFs were analyzed by 12.5%SDS-PAGE. Dominant bands which consist of four different band in the ranges of 85-97 , 55-65, 30-36 and 20-24 KDa showed similarities between sheep and human. To determine antigenic characteristics of bands one sample of human HF and two samples of sheep HF were used for Dot blotting and also Western blotting. In this research sera were taken from healthy and infected sheep and human. The results showed that color reactions were observed on the nitrocellulose paper in all of the mentioned sera in 20-24 KDa band on human (HF). No reaction on sheep samples were shown. These experiments showed different structures in humoral immunity between sheep and human.

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