Tissue engineering has opened up possibilities, for improving and modernizing treatment approaches for common illnesses [1]. Within tissue engineering, three primary parts are scaffold, cell, and biological factor [2]. Scaffolds provide a biocompatible substrate and architectural support for cell attachment and growth [3]. In this research, we prepared a scaffold using a combination of thermoplastic polyurethane (TPU), chitosan (Cs), and sodium alginate, which is a unique blend has not been utilized previously in vascular tissue engineering. The study aimed to create a scaffold for tissue engineering through a technique involving solvent casting. A solution of TPU in tetrahydrofuran (THF), Cs, and sodium alginate in 90% acetic acid was stirred for 12 hours. After mixing the solutions, samples were homogenized for 30 minutes using an ultrasonic bath. Scaffolds were sterilized by 70% ethanol and UV irradiation on each side of the surface for 20 min and incubated with the culture medium for 12 h. The biocompatibility of the scaffold was confirmed through MTT testing with 104 Human Umbilical Vein Endothelial Cell (HUVEC) cells per well in 96 well culture plate. Through the investigations for five days, It was found that the scaffold was biocompatible. The contact angle was 57°, which indicated the scaffold was highly hydrophilic, promoting cell attachment and growth. Scaffold prepared in this research exhibits biocompatibility and features a hydrophilic surface that encourages cell adhesion and growth. To make the scaffold specific for use in vascular tissue engineering, it is advised to use compounds with anticoagulant properties, such as heparin.

Keywords