The Effect of Eight Weeks of High-Intensity Interval Training along with Leucine Supplementation on Antioxidant Enzyme Levels in the Hippocampal Tissue of aged Male Wistar Rats پذیرفته شده برای ارائه شفاهی شناسه دیجیتال (DOI): 10.22089/ssrc.2024.4118 کد مقاله : 1057-SSRC نویسندگان فاطمه علی پور1 ، محمد مسافری ضیاءالدینی 2 ، سید علیرضا حسینی کاخک3 1دانشگاه فردوسی 2گروه فیزیولوژی ورزشی، دانشکده علوم ورزشی، دانشگاه فردوسی مشهد 3دانشگاه فردوسی مشهد چکیده Background: Aging is a physiological condition with unknown causes, likely related to a multifactorial process involving decreased endogenous antioxidant production, increased free radicals, and oxidative stress indices. This study aimed to compare the effect of eight weeks of high-intensity interval training (HIIT) and leucine supplementation on the levels of antioxidant enzymes in the hippocampal tissue of elderly male Wistar rats. Methods: This experimental study included 32 male Wistar rats (21 months old, weighing 390±12g), randomly divided into four groups: HIIT, leucine supplement, HIIT with leucine supplement, and control. The HIIT program lasted eight weeks, with three sessions per week and a 48-hour interval between sessions. The training program included running on a treadmill at a speed of 36m/min for 2 minutes and active rest at 20m/min for 3 minutes, increasing from 4 to 9 intervals by the end of the training protocol. In the supplement and supplement+exercise groups, 1.5g of leucine per rat was dissolved daily in water at 70°C. Hippocampal tissue was carefully separated 48 hours after the last training session, frozen in liquid nitrogen, and transferred to a -80°C freezer. SOD and CAT levels were measured using spectrophotometry. Results: The results showed that HIIT significantly increased SOD levels and decreased MDA (P<0.05). However, leucine supplementation decreased SOD and CAT levels and increased MDA and TAC levels (P<0.05). These results were also observed in the HIIT group with leucine supplementation. Conclusion: The results of this study showed that eight weeks of HIIT could enhance the antioxidant system in hippocampal tissue, while leucine supplementation increased oxidative stress indices. However, leucine supplementation along with HIIT reduced oxidative stress indices and increased total antioxidant levels in the hippocampus of elderly rats.

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