Toxicology in vitro, ( ISI ), Volume (101), Year (2024-12) , Pages (105924-105936)

Title : ( Assessment of ursolic acid effect on in vitro model of cardiac fibrosis )

Authors: Samane Alsadat Hosseiny , zahra esmaeili , Zeinab Neshati ,

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Abstract

This study aimed to evaluate the effects of ursolic acid (UA) on Angiotensin II (Ang II)-treated neonatal rat cardiac fibroblasts (rCFs) as an in vitro model of cardiac fibrosis. The rCFs were isolated from two-day-old neonatal rats. An in vitro model of cardiac fibrosis was established using 500 nm Ang II treatment for 48 h. The cells were then treated with 5 and 10 μM of UA for 24 and 48 h. Masson s trichrome staining, hydroxyproline content assay, scratch assay, apoptosis assay, measurements of superoxide dismutase (SOD) and malondialde-hyde (MDA) levels, real-time PCR, immunocytology and western blotting, were employed to assess the impact of UA. Ang II induced fibrosis in rCFs, as evidenced by the examination of various fibrotic markers. Upon treatment with 5 and 10 μM of UA, the amount of fibrosis in Ang II-treated rCFs was significantly decreased, so that the hydroxyproline concentration was reduced to 0.3 and 0.7 times, respectively. The RNA expression of the Col1a1,Col3a1, Tgfb1, Acta2 and Mmp2 genes had a decrease as well as Nrf2 and HO-1 had an increase after UA treatment. UA could lessen the harmful effects of cardiac fibrosis in a dose- and time-dependent manner, due to its antiapoptotic, antioxidant and cardioprotective properties. This suggests the potential of UA for treatment of cardiac fibrosis.

Keywords

, Cardiac fibrosis, Neonatal rat cardiac fibroblasts Angiotensin II, Ursolic acid
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@article{paperid:1100958,
author = {Hosseiny, Samane Alsadat and Esmaeili, Zahra and Neshati, Zeinab},
title = {Assessment of ursolic acid effect on in vitro model of cardiac fibrosis},
journal = {Toxicology in vitro},
year = {2024},
volume = {101},
month = {December},
issn = {0887-2333},
pages = {105924--105936},
numpages = {12},
keywords = {Cardiac fibrosis; Neonatal rat cardiac fibroblasts Angiotensin II; Ursolic acid},
}

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%0 Journal Article
%T Assessment of ursolic acid effect on in vitro model of cardiac fibrosis
%A Hosseiny, Samane Alsadat
%A Esmaeili, Zahra
%A Neshati, Zeinab
%J Toxicology in vitro
%@ 0887-2333
%D 2024

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