The g-C3N4 (g-CN) thin film proved to be effective in decreasing bacterial resistance. The g-CN thin films were prepared from g-CN powder by a simple and reproducible electrophoretic deposition (EPD) method. The thin films were deposited using g-CN powders with positive charges on the stainless steel (Ss) mesh as the substrate by applying a high voltage. Five synthesized powders with different calcination times were used to prepare various films under the same sonication conditions and EPD performance. The thin film prepared from g-CN powder in a 3 h calcination time displayed the highest antibacterial activity against Acinetobacter baumannii (A. baumannii). For this powder, different films were formed by varying the time of deposition. XRD, BET, AFM, and SEM analyses were used to characterize the films. The results of the live-dead assay, protein/nucleic acid leakage analysis, and SEM imaging confirmed a loss of cell membrane integrity, with a more pronounced effect observed for the g-CN thin film compared to the Ss mesh. BET and AFM analyses were obtained for specific surface area and surface roughness, 248.9 m²/g and Ra = 490, Rq = 570 nm, respectively, for optimum thin film. The obtained results have demonstrated that the g-CN thin film sample prepared by powder in 3 h calcination with an electrodeposition time of 20 min had the best performance and antibacterial effect.

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