In this study, the effect of magnetized solvent on the synthesis and properties of magnetic graphene oxide@UiO-66 (MGO@UiO-66) nanocomposite as sorbent was investigated. The prepared sorbent was used for the magnetic solid-phase extraction (MSPE) of remdesivir drug from water and plasma samples. The extracted remdesivir was quantitatively determined by high-performance liquid chromatography with a photodiode array detector (HPLC-PDA). The MGO@UiO-66 sorbents were characterized using various techniques such as Fourier-transform infrared spectroscopy (FT-IR), field emission scanning electron microscopy (FE-SEM), Brauer-emmett-teller analysis (BET), transmission electron microscopy (TEM), energy-dispersive X-ray spectroscopy (EDX) and vibrating-sample magnetometry. The optimization of extraction parameters such as desorption solvent and its volume, sorbent amount, pH, desorption, and extraction time were investigated and studied. Under the optimized conditions, the calibration plot for remdesivir was linear within the range of 0.1–300 ng mL−1 and limits of detection (0.03 ng mL–1). The MSPE-HPLC-PDA method showed good extraction recovery and relative standard deviations (RSD%) in the range of 95.5–99.5% and 3.8–4.7%, respectively. Furthermore, the method that was effectively utilized to analyze remdesivir in water and plasma samples, resulted in relative recoveries ranging from 95.5% to 99.3%.

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